Uniform serial sectioning for transmission electron microscopy.

Serial section scanning electron microscopy of adult brain tissue using focused ion beam milling.

Introduction Analyzing the synaptic basis of neuronal circuits within a volume of brain tissue requires electron microscopy. With a resolution capable of seeing the smallest syn-aptic contacts, this method uses different sectioning techniques to produce serial images suitable for seeing the ultrastruc-ture within significant volumes of brain tissue. Sections are cut from a block of tissue, leav...

Serial Section Scanning Electron Microscopy (S3EM) on Silicon Wafers for Ultra-Structural Volume Imaging of Cells and Tissues

High resolution, three-dimensional (3D) representations of cellular ultrastructure are essential for structure function studies in all areas of cell biology. While limited subcellular volumes have been routinely examined using serial section transmission electron microscopy (ssTEM), complete ultrastructural reconstructions of large volumes, entire cells or even tissue are difficult to achieve u...

Topics: 3D CLSM and Light Microscopy, 3D FIB/SEM or Serial Sectioning time resolved microscopy, 3D Image Analysis and simulation 3D TEM Tomography and Serial Sectioning, 3D X-ray Microscopy and Tomography, Ultra-Resolution Tomography Scientific committee and chairpersons:

Dislocation injection in strontium titanate by femtosecond laser pulses

Articles you may be interested in Electron backscatter diffraction on femtosecond laser sulfur hyperdoped silicon Appl. Comparison of heat-affected zones due to nanosecond and femtosecond laser pulses using transmission electronic microscopy Appl. Femtosecond laser ablation is used in applications which require low damage surface treatments, such as serial sectioning, spectroscopy, and micromac...

Serial Block-Face Scanning Electron Microscopy to Reconstruct Three-Dimensional Tissue Nanostructure

Three-dimensional (3D) structural information on many length scales is of central importance in biological research. Excellent methods exist to obtain structures of molecules at atomic, organelles at electron microscopic, and tissue at light-microscopic resolution. A gap exists, however, when 3D tissue structure needs to be reconstructed over hundreds of micrometers with a resolution sufficient...